Green tea compound can help slip therapeutics inside cells

| | Beijing
  • 0

Green tea compound can help slip therapeutics inside cells

Thursday, 20 September 2018 | PTI | Beijing

Green tea compound can help slip therapeutics inside cells

Scientists have found that an antioxidant found in green tea can be used to 'sneak in' therapeutic compounds into cells, and can be used to tackle

Drinking green tea has been linked to health benefits ranging from cardiovascular disease prevention to weight loss.

Although many of these claims still need to be verified in the clinic, an antioxidant in green tea called epigallocatechin gallate (EGCG) appears to have beneficial effects in cells and animals.

Researchers from South China University of Technology and East China Normal University in China have found that EGCG can be used to sneak therapeutic RNAs into cells.

Small interfering RNAs (siRNAs) have great therapeutic potential because they can dial down the expression of disease-related genes. However, getting siRNAs into cells where they can do their job has been challenging.

Being relatively large and negatively charged, siRNAs cannot easily cross the cell membrane, and they are susceptible to degradation by RNA-chomping enzymes.

To overcome these problems, some researchers have tried coating siRNAs with various polymers. However, most small polymers can't shuttle siRNAs into cells, whereas larger polymers can be effective but are generally toxic.

Researchers wondered if they could use EGCG, which is known to bind strongly to RNA, in combination with a small polymer to form nanoparticles that safely deliver siRNA into cells.

They made their nanoparticles by first combining EGCG and siRNA, which self-assembled into a negatively charged core. Then, the researchers coated this core with a shell consisting of a small, positively charged polymer.

These nanoparticles efficiently knocked down the expression of several target genes in cultured cells, showing that the particles could cross the cell membrane.

Next, the researchers tested the nanoparticles in a mouse model of intestinal injury, using an siRNA that targeted a pro-inflammatory enzyme.

The nanoparticles improved symptoms such as weight loss, shortening of the colon and intestinal inflammation. In addition to the gene-silencing effects of the siRNA, EGCG could contribute to the nanoparticles' effectiveness through its antioxidant and anti-inflammatory properties, researchers said.

 

 

Sunday Edition

CAA PASSPORT TO FREEDOM

24 March 2024 | Kumar Chellappan | Agenda

CHENNAI EXPRESS IN GURUGRAM

24 March 2024 | Pawan Soni | Agenda

The Way of Bengal

24 March 2024 | Shobori Ganguli | Agenda

The Pizza Philosopher

24 March 2024 | Shobori Ganguli | Agenda

Astroturf | Lord Shiva calls for all-inclusiveness

24 March 2024 | Bharat Bhushan Padmadeo | Agenda

Interconnected narrative l Forest conservation l Agriculture l Food security

24 March 2024 | BKP Sinha/ Arvind K jha | Agenda